Isolation of a cDNA copy of an RNA species expressed in murine pre-B cells

摘要: A part of a gene, pZ 183, was isolated from cDNA library constructed poly(A)-containing RNA the chemically induced murine pre-B lymphoma 70Z/3. This DNA sequence found to be present in one 5,000 clones unsubtracted library, and 350 which sequences common 70Z/3 T cell hybridoma had been subtracted. indicates that corresponding gene belongs genes are expressed with low medium abundance line The lymphomas, but not B myelomas, macrophage tumors or fibroblast line. By situ hybridization it detected 2-4% fetal liver cells between day 15 16 gestation, 1% adult bone marrow cells. It thymus cells, nor mature spleen, liver, kidney, heart, lung brain. size transcript all lymphomas is 1.2 kb length. 183 would appear useful B-lineage specific marker identifies at various stages before cell.