The proteome of Mycoplasma genitalium

摘要: Mycoplasma genitalium is the smallest member of class Mollicutes, with a genome size 580 kb. It has potential to express 480 gene products, and therefore considered be an excellent model assess: (a) minimum metabolism required by free living cell; (b) proteomic technologies information obtained proteome analysis. Here, we report on most complete observed at 73% (expected proteome), analysed 33% (reported proteome). The use four overlapping pH windows in conjunction SDS/PAGE allowed 427 distinct proteins resolved association exponential growth M. genitalium. Proof expression for 201 sufficient abundance silver stained two-dimensional gels was using peptide mass fingerprinting (PMF) which 158 were identified. product modification even simplest known self-replicating organism quantified ratio 1.22 : 1, more than genes. A reduction protein 42% post-exponentially-grown cells. DnaK, GroEL, DNA gyrase, cytadherence accessory significantly elevated, while some ribosomal reduced relative abundance. strengths weaknesses techniques employed assessed respect predicted derived from sequence information. Proteomics shown provide perspective into biochemical metabolic activities this organism, beyond that obtainable sequencing alone.