Synthetic Nucleotides as Probes of DNA Polymerase Specificity

摘要: The genetic code is continuously expanding with new nucleobases designed to suit specific research needs. These synthetic nucleotides are used study DNA polymerase dynamics and specificity may even inhibit activity. availability of an increasing chemical diversity allows questions utilization by different polymerases be addressed. Much the work in this area deals A family polymerases, for example, Escherichia coli I, which involved replication whose fidelity relatively high, but more recent includes other families including Y family, members known error prone. This paper focuses on ability utilize nonnatural templates or as incoming nucleoside triphosphates. Beyond utility probes specificity, such entities can also provide insight into functions when encountering that damaged natural agents. Thus, how deal well mutagenic potential nucleotides.