In vivo induction of increased DNA ploidy of mouse cervicovaginal epithelium by neonatal estrogen treatment.

摘要: The purpose of this study was to test the hypothesis that exposure natural estrogen early in development hormonedependent tissue induces a change nuclear DNA content. Female BALB/c mice were treated neonatally with daily s.c. injections either 25 Ig 170-estradiol (E,) 0.02 ml sesame oil (vehicle) or vehicle alone for 5 days. Treatment begun within 15 h birth 6 days after birth. One set each 10-day-old E,-treated and control received pellet implants containing mg E, cholesterol (10% 90% cholesterol), second alone, third did not receive implants. Cervicovaginal tracts from intact examined histologically by flow cytometry at 21, 40, 70, 180, 240 age. results obtained include several important findings: 1) neonatal treatment causes an increase content cervicovaginal epithelium; 2) short-term administration secondary exogenous E2 reduces latency period appearance increased 3) can indicate abnormal epithelium before histological abnormalities become evident; 4) there is sensitive induction epithelium. These findings support other reports carcinogenic potential vivo. Therefore, ploidy may be detectable event estrogen-induced carcinogenesis.