Cyclo(phenylalanine-proline) induces DNA damage in mammalian cells via reactive oxygen species.

摘要: Cyclo(phenylalanine-proline) is produced by various organisms such as animals, plants, bacteria and fungi. It has diverse biological functions including anti-fungal activity, anti-bacterial activity molecular signalling. However, a few studies have demonstrated the effect of cyclo(phenylalanine-proline) on mammalian cellular processes, cell growth apoptosis. In this study, we investigated whether affects responses associated with DNA damage in cells. We found that treatment 1 mM induces phosphorylation H2AX (S139) through ATM-CHK2 activation well double strand breaks. Gene expression analysis revealed subset genes related to regulation reactive oxygen species (ROS) scavenging production suppressed treatment. also perturbation mitochondrial membrane, resulting increased ROS, especially superoxide, production. Collectively, our study suggests via elevation ROS Our findings may help explain mechanism underlying bacterial infection-induced response host