Reduced antioxidant level and increased oxidative damage in intact liver lobes during ischaemia-reperfusion

摘要: AIM: To determine whether increased blood flow of the liver can cause oxidative stress and hepatocyte damage, to elaborate methods suitable for measuring antioxidant defence during hepatic surgery on rat model. METHODS: In nembutal narcosis, left lateral medial lobes were clipped 45 min make total supply through other lobes. Total status, glutathione peroxidase superoxide dysmutase activity, as well concentrations diene conjugates free sulphydril groups, H-donating ability reducing power samples determined. Chemiluminescent intensity was also measured. Metal ions (Al, Ca, Cu, Fe, Mg, Mn, Zn) P S determined with an inductively coupled plasma optical emission spectrometer Se content measured by cathodic stripping voltammetry. RESULTS: Glutathione activities decreased significantly in hyperemia group compared those observed sham operated group. The level status lower ability, concentration showed same tendency. A significant correlation (P<0.05) found between changes non-specific activities. This pointed simultaneous activity system. Al, Zn, than when levels higher hyperemia. CONCLUSION: Oxidative is one main factors injury intact ischaemia-reperfusion.