Purification and characterization of a proteinase inhibitor from white croaker skeletal muscle (Micropogon opercularis).
作者： M.P Sangorrı́n , E.J Folco , C.M Martone , J.J Sánchez
DOI: 10.1016/S1357-2725(01)00054-1
关键词: Serine Proteinase Inhibitors 、 Trypsin inhibitor 、 Kunitz STI protease inhibitor 、 Proteases 、 Trypsin 、 Serine protease 、 Proteinase 3 、 Protease 、 Molecular biology 、 Biochemistry 、 Biology
摘要: A trypsin proteinase inhibitor has been purified to homogeneity from the skeletal muscle of white croaker (Micropogon opercularis). Previously, we had described occurrence in fish a serine protease (proteinase I) which showed great capacity degrade whole myofibrils vitro and an endogenous that prevented action protease, both on natural artificial substrates. In this paper, report purification further biochemical characterization inhibitor. The was carried out by DEAE-Sephacel, Con A-Sepharose, Sephacryl S-300 Mono Q. Throughout procedure, inhibitory activity assayed using azocasein as substrate. molecular mass 65 kDa, estimated SDS-PAGE gel filtration. is glycoprotein, deduced fact it binds A-Sepharose stains with PAS wide range pH stability (from 5 11). thermal considerably decreased at temperatures >60 degrees C. Assays against various proteases indicated highly specific for proteases, since did not inhibit belonging any other groups. able target enzyme dose-dependent manner, 50% inhibition molar ratio close 1. present work contributes improving our understanding physiological role I-inhibitor system protein breakdown, well its influence post mortem proteolysis.
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sci-hub.se 参考文章(37)
E J E Folco, L Busconi, C B Martone, J J Sánchez, Fish skeletal muscle contains a novel serine proteinase with an unusual subunit composition. Biochemical Journal. ,vol. 263, pp. 471- 475 ,(1989) , 10.1042/BJ2630471
N. F. Haard, Protein hydrolysis in seafoods Springer, Boston, MA. pp. 10- 33 ,(1994) , 10.1007/978-1-4615-2181-5_3
E G Krebs, L Waxman, Identification of two protease inhibitors from bovine cardiac muscle. Journal of Biological Chemistry. ,vol. 253, pp. 5888- 5891 ,(1978) , 10.1016/S0021-9258(17)34549-0
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Michael G Thompson, Robert M Palmer, Signalling Pathways Regulating Protein Turnover in Skeletal Muscle Cellular Signalling. ,vol. 10, pp. 1- 11 ,(1998) , 10.1016/S0898-6568(97)00076-4
Haruhiko TOYOHARA, Yasuo MAKINODAN, Kazuyoshi TANAKA, Shizunori IKEDA, Detection of Calpastatin and a Trypsin Inhibitor in Carp Muscle Agricultural and biological chemistry. ,vol. 47, pp. 1151- 1154 ,(1983) , 10.1271/BBB1961.47.1151
L. Busconi, E.J. Folco, C. Martone, R.E. Trucco, J.J. Sánchez, Identification of two alkaline proteases and a trypsin inhibitor from muscle of white croaker (Micropogon opercularis) FEBS Letters. ,vol. 176, pp. 211- 214 ,(1984) , 10.1016/0014-5793(84)80943-6
Min-Jie Cao, Kiyoshi Osatomi, Risa Matsuda, Makoto Ohkubo, Kenji Hara, Tadashi Ishihara, Purification of a novel serine proteinase inhibitor from the skeletal muscle of white croaker (Argyrosomus argentatus). Biochemical and Biophysical Research Communications. ,vol. 272, pp. 485- 489 ,(2000) , 10.1006/BBRC.2000.2803
Lothar Kuehn, Burkhardt Dahlmann, Hans Reinauer, Identification of four distinct serine proteinase inhibitors in rat skeletal muscle Biochemical and Biophysical Research Communications. ,vol. 120, pp. 96- 102 ,(1984) , 10.1016/0006-291X(84)91418-9
Kiyoshi Osatomi, Hiroshi Sasai, Minjie Cao, Kenji Hara, Tadashi Ishihara, Purification and Characterization of Myofibril-bound Serine Proteinase from Carp Cyprinus carpio Ordinary Muscle Comparative Biochemistry and Physiology B. ,vol. 116, pp. 183- 190 ,(1997) , 10.1016/S0305-0491(96)00208-8