Evidence for the involvement of a reperfusion injury in galactosamine/endotoxin-induced hepatitis in mice

摘要: Simultaneous intraperitoneal administration of 700 mg/kg galactosamine and 33 micrograms/kg Salmonella abortus equi endotoxin to male NMRI albino mice resulted in fulminant hepatitis as assessed after nine hours by measurement serum transaminases well sorbitol dehydrogenase activities. Intraperitoneal pretreatment animals with 2 X 100 allopurinol, or intravenous kU superoxide dismutase 1 MU catalase fully prevented hepatitis. Administration 10 the prostacyclin analogue iloprost antagonized liver injury when given simultaneously galactosamine/endotoxin but did not protect 90 min later. Tocopherol desferal had no significant protective effect. Together our recent finding that hepatic leukotriene D4 production is likely be responsible for galactosamine/endotoxin-induced we interpret these results evidence a leukotriene-induced ischemia followed reperfusion syndrome.