作者: Svetlana I Galkina , Natalia V Fedorova , Alexander L Ksenofontov , Marina V Serebryakova , Ekaterina A Golenkina
关键词: Actin cytoskeleton 、 Cell biology 、 Cell adhesion 、 Hydroxylysine 、 Lysyl hydroxylase 、 Chemistry 、 Matrix metalloproteinase inhibitor 、 Wortmannin 、 Extracellular matrix 、 Fibronectin
摘要: During infection or certain metabolic disorders, neutrophils can escape from blood vessels, invade and attach to other tissues. The invasion adhesion of is accompanied maintained by their own secretion. We have previously found that fibronectin dramatically selectively stimulates the release free amino acid hydroxylysine. role hydroxylysine lysyl hydroxylase in neutrophil has not been studied, nor processes control them. Using analysis, mass spectrometry electron microscopy, we inhibitor minoxidil, matrix metalloproteinase doxycycline, PI3K/Akt pathway inhibitors wortmannin Akt1/2 drugs affect actin cytoskeleton significantly block partially completely suppress spreading neutrophils. effectors Akt 1/2 also increase phenylalanine release. hypothesize upon result activation interaction with metalloproteinase, intact cytoskeleton, which play important roles recruitment into tissue through extracellular remodeling.