Neutrophil Adhesion and the Release of the Free Amino Acid Hydroxylysine

作者: Svetlana I Galkina , Natalia V Fedorova , Alexander L Ksenofontov , Marina V Serebryakova , Ekaterina A Golenkina

DOI: 10.3390/CELLS10030563

关键词: Actin cytoskeletonCell biologyCell adhesionHydroxylysineLysyl hydroxylaseChemistryMatrix metalloproteinase inhibitorWortmanninExtracellular matrixFibronectin

摘要: During infection or certain metabolic disorders, neutrophils can escape from blood vessels, invade and attach to other tissues. The invasion adhesion of is accompanied maintained by their own secretion. We have previously found that fibronectin dramatically selectively stimulates the release free amino acid hydroxylysine. role hydroxylysine lysyl hydroxylase in neutrophil has not been studied, nor processes control them. Using analysis, mass spectrometry electron microscopy, we inhibitor minoxidil, matrix metalloproteinase doxycycline, PI3K/Akt pathway inhibitors wortmannin Akt1/2 drugs affect actin cytoskeleton significantly block partially completely suppress spreading neutrophils. effectors Akt 1/2 also increase phenylalanine release. hypothesize upon result activation interaction with metalloproteinase, intact cytoskeleton, which play important roles recruitment into tissue through extracellular remodeling.

参考文章(63)
Svetlana I. Galkina, Natalia V. Fedorova, Marina V. Serebryakova, Evgenii A. Arifulin, Vladimir I. Stadnichuk, Tatjana V. Gaponova, Ludmila A. Baratova, Galina F. Sud'ina, Inhibition of the GTPase dynamin or actin depolymerisation initiates outward plasma membrane tubulation/vesiculation (cytoneme formation) in neutrophils Biology of the Cell. ,vol. 107, pp. 144- 158 ,(2015) , 10.1111/BOC.201400063
Saran Feng, Jiannong Cen, Yihong Huang, Hongjie Shen, Li Yao, Yuanyuan Wang, Zixing Chen, Matrix metalloproteinase-2 and -9 secreted by leukemic cells increase the permeability of blood-brain barrier by disrupting tight junction proteins. PLOS ONE. ,vol. 6, ,(2011) , 10.1371/JOURNAL.PONE.0020599
Svetlana I. Galkina, Natalia V. Fedorova, Marina V. Serebryakova, Julia M. Romanova, Sergei A. Golyshev, Vladimir I. Stadnichuk, Ludmila A. Baratova, Galina F. Sud'ina, Thomas Klein, Proteome analysis identified human neutrophil membrane tubulovesicular extensions (cytonemes, membrane tethers) as bactericide trafficking Biochimica et Biophysica Acta. ,vol. 1820, pp. 1705- 1714 ,(2012) , 10.1016/J.BBAGEN.2012.06.016
Chunguang Wang, Marja-Maija Ristiluoma, Antti M. Salo, Sinikka Eskelinen, Raili Myllylä, Lysyl hydroxylase 3 is secreted from cells by two pathways Journal of Cellular Physiology. ,vol. 227, pp. 668- 675 ,(2012) , 10.1002/JCP.22774
Toshiaki Sato, Yulong Li, Tomoaki Saito, Haruaki Nakaya, Minoxidil opens mitochondrial KATP channels and confers cardioprotection British Journal of Pharmacology. ,vol. 141, pp. 360- 366 ,(2004) , 10.1038/SJ.BJP.0705613
S. Murad, L.C. Walker, S. Tajima, S.R. Pinnell, Minimum structural requirements for minoxidil inhibition of lysyl hydroxylase in cultured fibroblasts Archives of Biochemistry and Biophysics. ,vol. 308, pp. 42- 47 ,(1994) , 10.1006/ABBI.1994.1006
Chunguang Wang, Vuokko Kovanen, Päivi Raudasoja, Sinikka Eskelinen, Helmut Pospiech, Raili Myllylä, The glycosyltransferase activities of lysyl hydroxylase 3 (LH3) in the extracellular space are important for cell growth and viability. Journal of Cellular and Molecular Medicine. ,vol. 13, pp. 508- 521 ,(2009) , 10.1111/J.1582-4934.2008.00286.X
Eline Dejonckheere, Roosmarijn E. Vandenbroucke, Claude Libert, Matrix metalloproteinases as drug targets in ischemia/reperfusion injury. Drug Discovery Today. ,vol. 16, pp. 762- 778 ,(2011) , 10.1016/J.DRUDIS.2011.06.009
Elena I. Deryugina, James P. Quigley, Matrix metalloproteinases and tumor metastasis Cancer and Metastasis Reviews. ,vol. 25, pp. 9- 34 ,(2006) , 10.1007/S10555-006-7886-9