Effects of testosterone and 17β-estradiol on osteogenic and adipogenic differentiation capacity of human bone-derived mesenchymal stromal cells of postmenopausal women.

摘要: Progressive bone loss is a predominant symptom of aging and osteoporosis. Therefore, the effects sex steroids (i.e. testosterone 17β-estradiol) on differentiation capacity human bone-derived mesenchymal stromal cells (hMSCs), as progenitors osteoblasts adipocytes, are particular interest. The objectives present study were, thus, to elucidate whether hMSCs postmenopausal women produce aromatase (CYP19A1) and, they modulate their behaviour in response 17β-estradiol (E2), relation steroid receptor expression. Supplementation resulted considerable formation E2 under osteogenic adipogenic culture conditions, whereas synthesis remained minimal cultured basal medium. Concomitant with high expression medium supplemented testosterone, distinct promotion late-stage osteogenesis was found, shown by significant matrix mineralization notable increase markers. These were abrogated inhibitor anastrozole. Under reduced occurrence lipid droplets led decrease PPARγ AR expression, independent Regardless ERα detectable whilst ERβ not. In conclusion, activity limited differentiated resulting enhances late stages via ERα. Adipogenic differentiation, other hand, both steroids: 17β-estradiol.