Isolation and overexpression in Escherichia coli of the flavodoxin gene from Anabaena PCC 7119.

作者： M F Fillat , W E Borrias , P J Weisbeek

关键词: Gene expression 、 Flavodoxin 、 Anabaena 、 Peptide sequence 、 Biology 、 Recombinant DNA 、 Expression vector 、 Escherichia coli 、 Molecular biology 、 Gene

摘要: The gene coding for flavodoxin from Anabaena PCC 7119 was cloned by using the polymerase chain reaction (PCR). is transcribed into a 1250-base transcript. expression of analysed and found to be regulated at transcriptional level availability iron. PCR-amplified vector pTrc 99b expressed in Escherichia coli. High concentrations were (20% total protein). recombinant protein purified cytosolic fraction cells it exhibited properties identical with those wild-type flavodoxin.