Signaling aptamers created using fluorescent nucleotide analogues.

摘要: A new approach to creating fluorescent signaling aptamers using nucleotide analogues is presented. The fluorescence quantum yield of such as 2-aminopurine strongly depends on base stacking interactions when incorporated into double or single stranded DNA. This property used generate a binding-specific signal. Aptamers for human α-thombin, immunoglobulin E, and platelet-derived growth factor B were modified with in positions that undergo conformational changes. resulting show specific, binding-induced increase the signal up 30-fold. Conformation-changing these identified by screening set aptamer sequences each included analogue at different position. modifications estimated modeling secondary structure. It likely this producing si...