Rapid Separation of Milk Whey Proteins by Anion Exchange Chromatography

摘要: A fast protein liquid chromatography system was used to fractionate the major proteins of sweet and acid wheys. Fifty 500 microliter whey were fractionated with a stepwise ionic strength gradient using water (buffer A) increasing concentrations .7 M sodium acetate B). Six well-resolved peaks obtained: 1) amino acids (tentative identification), 2) low molecular weight peptides 3) highly enriched alpha-lactalbumin, 4) serum albumin 5) electrophoretically pure beta-lactoglobulin B, A. poor baseline or unresolved resulted when .02 bis-tris histidine for buffer in B. When chloride place acetate, beta-lactoglobulins B poorly resolved. The column cleaned after each run by injecting 2 ml following reagents: glacial acetic acid, N chloride, hydroxide, 2% detergent, 100% acetonitrile. Time required sample including cleanup 40 min.