Kindling status in sprague-dawley rats induced by pentylenetetrazole: involvement of a critical development period.

摘要: Kindled seizures are widely used as a model for epileptogenesis. Although the achievement of kindling criterion is known to require time develop, precise developmental period has not been identified. We now report that optimal in Sprague-Dawley rat associated with critical inter-stimulus interval 24 26 days. show highly efficient can be achieved only two subconvulsive doses pentylenetetrazole so long they given 25 days apart. Using Northern blot hybridization we increased seizure susceptibility at coincides an expression plasticity-associated proteins, growth-associated protein-43 (GAP-43), microtubule-associated protein 1B (MAP1B), and tissue plasminogen activator (tPA) mRNAs hippocampus. By situ immunocytochemistry on sections, also GAP-43 polymorphic layer dentate gyrus, mossy fibers, pyramidal cells CA3 region The demonstration long, defined inducing should enable dissection cellular genetic events underlying this phenomenon rat.