Comparison of genomic and antimicrobial resistance features of latex agglutination test-positive and latex agglutination test-negative Staphylococcus aureus isolates causing bovine mastitis

摘要: The dairy industry suffers massive economic losses due to staphylococcal mastitis in cattle. Staphaureux latex agglutination test (Oxoid, Basel, Switzerland) was reported lead negative results 54% of bovine Staphylococcus aureus strains, and latex-negative strains are thought be less virulent than Staphaurex latex-positive strains. However, comparative information on virulence resistance profiles these 2 groups Staph. is scarce. Our objective associate the phenotype isolated from milk with data clonal complexes, genes, antibiotic (1) determine positive groups, (2) provide needed improve treatment identify potential vaccine targets. Seventy-eight 78 cows 57 Swiss farms were characterized. Latex tested by kit, generated disk diffusion. A DNA microarray used assign complexes (CC) gene profiles. By test, 49% isolates 51% latex-negative. All assigned CC151, whereas various including CC97 (n=16), CC8 (n=10), CC479 (n=5), CC20 (n=4), CC7 (n=1), CC9 CC45 (n=1). Although susceptible all antimicrobial agents tested, 24% classified as intermediate regard cefalexin-kanamycin 13% resistant both ampicillin penicillin. Microarray highly similar, but differed largely those isolates. group lacked several enterotoxin genes sak, it exhibited significantly higher prevalence rates encoding C, toxic shock syndrome toxin, leukocidins (lukM/lukF-P83, lukD). findings suggest that represent a closely related specific patterns.