Motif for controllable toggle switch in gene regulatory networks

摘要: Abstract Toggle switch as a common phenomenon in gene regulatory networks has been recognized important for biological functions. Despite much effort dedicated to understanding the toggle and designing synthetic biology circuit achieve function, we still lack comprehensive of intrinsic dynamics behind such minimum structure that is imperative producing switch. In this paper, discover structure, motif enables controllable particular, consists transformative double negative feedback loop (DNFL) regulated by an additional driver node. By enumerating all possible configurations from node, identify two types motifs associated with captured existence bistable states. The sense gap between states adjustable determined strength nodes. We test effect self-oscillating network (SON) respect interplay other genes, find switching whole can be successfully controlled insofar contains single motif. Our findings are uncover underlying nonlinear have implications devising field biology.