作者: Mark E. Wells , John C. Vellinger , Mark S. Deuser , Kenneth W. Barton
DOI:
关键词: Low Gravity 、 Manifold (fluid mechanics) 、 Waste management 、 Sample collection 、 Peristaltic pump 、 Reactor pressure vessel 、 Suspension (chemistry) 、 Materials science 、 Bioreactor 、 Rotation
摘要: A bioreactor apparatus and cell culturing system is provided for the automated cultivation processing of living cells remotely both on earth in low gravity which utilizes a generally cylindrical reactor vessel that may be optionally rotated about its axis while allowing entrance fresh or recycled fluid removal, optionally, spent medium, medium to filtered unfiltered collection samples. method exchanging gases between culture ambient provided. fresh-medium storage bag peristaltic pump used batch feeding, perfusion sample collection. An enclosure manifold representing an additional level chemical containment series pinch valves periodic samples suspended cell-free disposed therein together with humidity control system. The computer controlled order all functions including rotation vessel, feeding perfusing timed from reactor, selecting collecting supernatant. sealed compartment sample-collection bags provides safety. external housing components device except power supply computer. loop electronic video microscope real-time recorded and/or transmitted observation suspension.