Open-top axially swept light-sheet microscopy
作者： Jung-Hoon Park , Ki Hean Kim , Euiheon Chung , Sunghoe Chang , Bumju Kim
DOI: 10.1364/BOE.419030
关键词: Light sheet fluorescence microscopy 、 Image resolution 、 Prism 、 Optical aberration 、 Lens (optics) 、 Optics 、 Microscopy 、 Field of view 、 Refractive index 、 Materials science
摘要: Open-top light-sheet microscopy (OT-LSM) is a specialized microscopic technique for high throughput cellular imaging of large tissue specimens including optically cleared tissues by having the entire optical setup below sample stage. Current OT-LSM systems had relatively low axial resolutions using weakly focused light sheets to cover field view (FOV). In this report, open-top axially swept LSM (OTAS-LSM) was developed high-throughput with improved resolution. OTAS-LSM tightly excitation sheet across FOV an electro tunable lens (ETL) and collected emission at focus camera in rolling shutter mode. air objective lenses liquid prism it on-axis aberration associated mismatch refractive indices between immersion medium. The effects were analyzed both simulation experiment, image under 1.6µm all directions. newly applied mouse brain small intestine, demonstrated single-cell resolution neuronal networks. might be useful examination tissues.
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