Genomic cloning, complete nucleotide sequence, and structure of the human gene encoding the major intrinsic protein (MIP) of the lens.
作者： M. Michele Pisano , Ana B. Chepelinsky
DOI: 10.1016/0888-7543(91)90023-8
关键词: Exon 、 Peptide sequence 、 Sequence alignment 、 Gene 、 Nucleic acid sequence 、 Intron 、 Alu element 、 Coding region 、 Genetics 、 Biology
摘要: Abstract Major intrinsic protein (MIP, also called MP26) is the predominant fiber cell membrane of ocular lens. MIP has been suggested to play a role in cell-cell communication Its expression tissue-specific and developmentally regulated. We have isolated characterized human gene encoding report here its genomic structure entire nucleotide sequence. The 3.6 kb, contains four exons separated by introns ranging size from 0.4 1.6 present single copy per haploid genome. Primer extension lens RNA indicates that transcription initiates site 26 nt downstream TATA box. Three complete Alu repetitive elements are found tandem 5′-flanking region gene, sequence third intron. interspecies comparisons coding homologies other members putative transmembrane channel superfamily discussed.
sci-hub.se 参考文章(39)
R S Sparkes, C Heinzmann, J Horwitz, M B Gorin, C A Jones, T Mohandas, M L Law, J B Bateman, The gene for the major intrinsic protein (MIP) of the ocular lens is assigned to human chromosome 12cen-q14. Investigative Ophthalmology & Visual Science. ,vol. 27, pp. 1351- 1354 ,(1986)
B L Smith, P Agre, Erythrocyte Mr 28,000 transmembrane protein exists as a multisubunit oligomer similar to channel proteins. Journal of Biological Chemistry. ,vol. 266, pp. 6407- 6415 ,(1991) , 10.1016/S0021-9258(18)38133-X
Larry J. Takemoto, Jeff S. Hansen, Bruce J. Nicholson, Michael Hunkapiller, Jean-Paul Revel, Joseph Horwitz, Major intrinsic polypeptide of lens membrane Biochimica et Biophysica Acta (BBA) - Biomembranes. ,vol. 731, pp. 267- 274 ,(1983) , 10.1016/0005-2736(83)90018-4
Stephen M. Mount, A catalogue of splice junction sequences Nucleic Acids Research. ,vol. 10, pp. 459- 472 ,(1982) , 10.1093/NAR/10.2.459
Michael B. Gorin, S.Barbara Yancey, Janice Cline, Jean-Paul Revel, Joseph Horwitz, The major intrinsic protein (MIP) of the bovine lens fiber membrane: Characterization and structure based on cDNA cloning Cell. ,vol. 39, pp. 49- 59 ,(1984) , 10.1016/0092-8674(84)90190-9
Graeme J. Wistow, M. Michele Pisano, Ana B. Chepelinsky, Tandem sequence repeats in transmembrane channel proteins. Trends in Biochemical Sciences. ,vol. 16, pp. 170- 171 ,(1991) , 10.1016/0968-0004(91)90065-4
R. Breathnach, C. Benoist, K. O'Hare, F. Gannon, P. Chambon, Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 4853- 4857 ,(1978) , 10.1073/PNAS.75.10.4853
G. M. Pao, L.-F. Wu, K. D. Johnson, H. Höfte, M. J. Chrispeels, G. Sweet, N. N. Sandal, M. H. Saier, Evolution of the MIP family of integral membrane transport proteins. Molecular Microbiology. ,vol. 5, pp. 33- 37 ,(1991) , 10.1111/J.1365-2958.1991.TB01823.X
A. Shiels, N. A. Kent, M. McHale, J. A. Bangham, Homology of MIP26 to Nod26 Nucleic Acids Research. ,vol. 16, pp. 9348- 9348 ,(1988) , 10.1093/NAR/16.19.9348
J. V. Maizel, R. P. Lenk, Enhanced graphic matrix analysis of nucleic acid and protein sequences. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 78, pp. 7665- 7669 ,(1981) , 10.1073/PNAS.78.12.7665