Molecular cloning of protease gene(s) from Xanthomanas campestris pv. campestris: Expression in Escherichia coli and role in pathogenicity
作者： J. L. Tang , C. L. Gough , C. E. Barber , J. M. Dow , M. J. Daniels
DOI: 10.1007/BF00327195
关键词: Genomic library 、 Subcloning 、 Structural gene 、 Molecular cloning 、 Cosmid 、 Genetics 、 Biology 、 Protease 、 Xanthomonas campestris 、 Escherichia coli
摘要: The recombinant plasmid pIJ3070 isolated from a genomic library of Xanthomonas campestris pv. constructed in the conjugal cosmid pLAFR3 contains protease gene(s) which can be expressed Escherichia coli. Tn5 mutagenesis and subcloning revealed that structural is(are) located ca. 10 kb EcoRI fragment. Several protease-minus mutants X. c. were obtained by marker exchange techniques. Studies pathogenicity these showed is not critically important for on turnip plants but may play minor role disease development.
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