Antileishmanial activity and ultrastructural changes of sesquiterpene lactones isolated from Calea pinnatifida (Asteraceae).
作者： Lhais Araujo Caldas , Meire L Yoshinaga , Marcelo JP Ferreira , Joao HG Lago , Adriana B de Souza
DOI: 10.1016/J.BIOORG.2018.10.059
关键词: Chemistry 、 Mechanism of action 、 Ultrastructure 、 Sesquiterpene 、 Biochemistry 、 Cytotoxicity 、 Miltefosine 、 Asteraceae 、 EC50 、 Amastigote
摘要: Abstract Bioactivity-guided fractionation of antileishmanial active CH2Cl2 phase MeOH extract from leaves Calea pinnatifida led to isolation two sesquiterpene lactones calein C (1) and calealactone (2), which structures were stablished on the basis spectroscopic analysis. Compounds 1 2 displayed potent activity against Leishmania amazonensis promastigotes with EC50 1.7 4.6 µg mL−1, respectively. Compound presented low cytotoxicity for J774 macrophages amastigote forms L. similar miltefosine CC50 values 31.73 27.18 µg mL−1, Additionally, compounds caused ultrastructural changes in leading a loss their classical structural morphology, as evidenced by electron microscopy. Also compound decreased mitochondria membrane potential. To best our knowledge, this is first occurrence C. pinnatifida. The results obtained highlighted importance studying isolated terms activity, order understand mechanism action amazonensis.
fapesp.br
sci-hub.se 参考文章(28)
Nádia Roque, Vitor Cavalcanti Carvalho, Estudos taxonômicos do gênero Calea (Asteraceae, Neurolaeneae) no estado da Bahia, Brasil Rodriguésia - Instituto de Pesquisas Jardim Botânico do Rio de Janeiro. ,vol. 62, pp. 547- 561 ,(2011) , 10.1590/2175-7860201162308
João Carvalho, Marchetti, Silva, Santos, Souza, Tinti, Figueira, Foglio, João Carvalho, The anticancer activity of dichloromethane crude extract obtained from Calea pinnatifida Journal of Experimental Pharmacology. ,vol. 4, pp. 157- 162 ,(2012) , 10.2147/JEP.S37135
Frederick C. Seaman, Sesquiterpene lactones as taxonomic characters in the asteraceae Botanical Review. ,vol. 48, pp. 121- 594 ,(1982) , 10.1007/BF02919190
Andréa Mendes do Nascimento, Dionéia C. R. de Oliveira, Marcos José Salvador, Regina Celia Candido, Sérgio de Albuquerque, Trypanocidal and antifungal activities of p-hydroxyacetophenone derivatives from Calea uniflora (Heliantheae, Asteraceae). Journal of Pharmacy and Pharmacology. ,vol. 56, pp. 663- 669 ,(2010) , 10.1211/0022357023231
Ferdinand Bohlmann, Jasmin Jakupovic, Neue germacranolide aus Calea urticifolia Phytochemistry. ,vol. 18, pp. 119- 123 ,(1979) , 10.1016/S0031-9422(00)90927-0
G. Odonne, G. Herbette, V. Eparvier, G. Bourdy, R. Rojas, M. Sauvain, D. Stien, Antileishmanial sesquiterpene lactones from Pseudelephantopus spicatus, a traditional remedy from the Chayahuita Amerindians (Peru). Part III. Journal of Ethnopharmacology. ,vol. 137, pp. 875- 879 ,(2011) , 10.1016/J.JEP.2011.07.008
Hankui Wu, Frank Fronczek, Charles Burandt, Jordan Zjawiony, Antileishmanial Germacranolides from Calea zacatechichi. Planta Medica. ,vol. 77, pp. 749- 753 ,(2011) , 10.1055/S-0030-1250584
Alexandre Rea, Andre G. Tempone, Erika G. Pinto, Juliana T. Mesquita, Eliana Rodrigues, Luciana Grus M. Silva, Patricia Sartorelli, João Henrique G. Lago, Soulamarin Isolated from Calophyllum brasiliense (Clusiaceae) Induces Plasma Membrane Permeabilization of Trypanosoma cruzi and Mytochondrial Dysfunction PLOS Neglected Tropical Diseases. ,vol. 7, ,(2013) , 10.1371/JOURNAL.PNTD.0002556
Zenaide S. Ferreira, Nídia F. Roque, Otto R. Gottlieb, Fernando Oliveira, Hugo E. Gottlieb, Structural clarification of germacranolides from Calea species Phytochemistry. ,vol. 19, pp. 1481- 1484 ,(1980) , 10.1016/0031-9422(80)80200-7
Sergio de Almeida Basano, Luís Marcelo Aranha Camargo, Leishmaniose tegumentar americana: histórico, epidemiologia e perspectivas de controle Revista Brasileira De Epidemiologia. ,vol. 7, pp. 328- 337 ,(2004) , 10.1590/S1415-790X2004000300010