MicroRNA‑217 inhibition relieves cerebral ischemia/reperfusion injury by targeting SIRT1
作者： Gaofeng Rao , Wenfu Zhang , Shegeng Song
关键词: Downregulation and upregulation 、 Sirtuin 1 、 Viability assay 、 Reperfusion injury 、 Proinflammatory cytokine 、 Oxidative stress 、 Apoptosis 、 Pharmacology 、 Oncogene 、 Medicine
摘要: MicroRNAs (miRs) have been proposed to be involved in the pathological processes of cerebral ischemia/reperfusion (CIR) injury. The present study aimed investigate potential role and molecular mechanisms miR‑217 regulation neuronal survival CIR To perform investigation, an vitro cellular model injury was established by treating neurons with oxygen‑glucose deprivation reoxygenation (OGD/R). levels were detected using reverse transcription‑quantitative PCR. association between sirtuin 1 (SIRT1) identified TargetScan validated a dual‑luciferase reporter assay. Cell viability apoptosis measured Counting Kit‑8 assay flow cytometry, respectively. release lactate dehydrogenase, production proinflammatory factors oxidative stress biomarkers analyzed ELISAs specific kits. It revealed that significantly upregulated OGD/R‑treated neurons. SIRT1 direct target miR‑217, downregulated following OGD/R treatment. Downregulation ameliorated OGD/R‑induced injury, inflammatory responses stress. effects inhibitor on treated attenuated knockdown. Additionally, western blotting SIRT1/AMP‑activated protein kinase‑α/NF‑κB pathway partially miR‑217. In conclusion, data indicated downregulation protected against targeting SIRT1.
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