ralGDS family members interact with the effector loop of ras p21.

摘要: Using a yeast two-hybrid system, we identified novel protein which interacts with ras p21. This shares 69% amino acid homology ral guanine nucleotide dissociation stimulator (ralGDS), GDP/GTP exchange for p24. We designated this RGL, ralGDS-like. the found that an effector loop mutant of p21 was defective in interacting p21-interacting domain suggesting binds to through Since ralGDS contained region highly homologous examined whether could interact In failed insect cells, made complex v-ras but not dominant negative interacted GTP-bound form GDP-bound vitro. inhibited both GTPase-activating activity neurofibromatosis gene product (NF1) and interaction Raf These results demonstrate specifically active can compete NF1 binding Therefore, family members may be proteins or inhibit interactions between its effectors.