Petal-like CdS nanospheres-based electrochemiluminescence aptasensor for detection of IgE with gold nanoparticles amplification

摘要: Abstract A facile label-free electrochemiluminescence (ECL) aptasensor was designed for sensitive detection of human immunoglobulin E (IgE) using petal-like CdS nanospheres and Au nanoparticles (AuNPs) as sensing platform. To construct the aptasensor, ECL emitter were firstly synthesized immobilized on chitosan-coated glassy carbon electrode (GCE) surface. Chitosan coated CdS/CS/GCE again with two coating numbers to produce a stable signal facilitate subsequent AuNPs immobilization. The construction achieved after IgE aptamer adsorbed onto AuNPs. performed upon incubation interface target protein IgE. Under optimum conditions, decreased depending linearly logarithmic value concentration ranging from 5.0 × 10−13 1.0 × 10−9 M regression equation I = −15254.8 − 2129.3 log c (R2 = 0.996). limit experimentally found be 8.0 × 10−14 M. applicability constructed demonstrated in determination serum samples.