Quantification of Campylobacter jejuni gene expression after successive stresses mimicking poultry slaughtering steps.

摘要: Broiler meat is considered as the most important source of foodborne pathogen Campylobacter jejuni. Exposure to stress conditions encountered during slaughtering process may induce bacterial adaptation mechanisms, and enhance or decrease resistance subsequent stress. This result from changes in gene expression. study aims accurately quantify expression selected C. jejuni genes after stresses inspired poultry process. RT-qPCR was used 44 three strains successive heat cold stresses. Main results indicated that 26 out were differentially expressed following thermal Three clusters according strain condition. Up-regulated mainly included involved shock response, whereas down-regulated belonged metabolic pathways (such lipid, amino-acid metabolisms). However, four similarly overexpressed strains; they might represent indicators response at species scale. Advances molecular understanding pathogenic bacteria, such Campylobacter, real-life processing will make it possible identify technological levers better mitigate microbial risk.