5-Hydroxytryptamine-induced synovial plasma extravasation is mediated via 5-hydroxytryptamine2A receptors on sympathetic efferent terminals.

摘要: 5-Hydroxytryptamine (5-HT) is known to act in peripheral tissues produce pain and inflammation, yet the mechanisms underlying 5-HT-induced inflammation have not been well studied. The present study uses a rat knee joint model of (synovial plasma extravasation) molecular biological techniques determine site action 5-HT specific receptor subtype mediating synovial extravasation. (1 microM) stimulates extravasation 7-fold above base-line levels. Surgical lumbar sympathectomy, but C-fiber depletion by neonatal capsaicin, dramatically reduces (P < .001), indicating that sympathetic efferents mediate this effect. Polymerase chain reaction amplification cDNA demonstrates 5-HT1A, 5-HT1B, 5-HT1D, 5-HT2A 5-HT3, 5-HT2C, subtypes are ganglia. With selective ligands for these subtypes, we demonstrate mediated via receptor. These findings suggest role antagonists various inflammatory states.