PATTERNS OF MESSENGER RNA EXPRESSION FOR alpha1-ADRENOCEPTOR SUBTYPES IN HUMAN CORPUS CAVERNOSUM
作者： JEAN-PIERRE DAUSSE , ALBERT LERICHE , FRANCOISE YABLONSKY
DOI: 10.1016/S0022-5347(01)62965-3
关键词: Messenger RNA 、 Optical density units 、 Biology 、 Complementary DNA 、 Alpha1 adrenoceptor 、 Dna amplification 、 Internal medicine 、 Gene expression 、 Endocrinology 、 Adrenergic receptor
摘要: AbstractPurpose: The present study evaluated the expression of alpha1-adrenoceptor subtypes in human corpus cavernosum.Materials and Methods: mRNA encoding alpha1a, alpha1b alpha1d were assessed by RNA-directed complementary cDNA synthesis followed Taq DNA amplification. level alpha1 was calculated arbitrary optical density units normalized with respect to length respective fragments.Results: We found that adrenoceptor are expressed cavernosum, a predominant alpha1a subtype.Conclusion: These results suggest alpha1a-adrenoceptor subtype is important understanding biochemical functional characteristics this may lead development specific antagonists treatment impotence.
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