Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages.

摘要: The tumor microenvironment is rich in multiple cell types that influence development. Macrophages infiltrate tumors, where they are the most abundant immune population and secrete a number of cytokines. Aspirin acts as chemopreventive agent against cancer This study investigated whether aspirin regulates crosstalk between breast cells macrophages. To these interactions microenvironment, conditioned media was employed using 4T1 cultured RAW 264.7 cell-conditioned medium (RAW-CM), cocultured model both used. When were RAW-CM, there increases viability secretion cytokines VEGF, PAI-1, TNF-α, IL-6. Treatment with inhibited growth migration MCP-1, IL-6 production. In coculture cells, IL-6, TGF-β. Furthermore, significantly decreased M2 macrophage marker CD206, but increased M1 CD11c expression. summary, treatment through regulation angiogenic inflammatory mediator production influenced M1/M2 subtype. highlighted suppresses favorable could be promising triple-negative cancer.