Characterization of HOG1 homologue, CpMK1, from Cryphonectria parasitica and evidence for hypovirus‐mediated perturbation of its phosphorylation in response to hypertonic stress

摘要: We examined the biological function of cpmk1, which encodes a MAPK Cryphonectria parasitica, and its regulation by mycovirus. Sequence comparisons revealed that cpmk1 had highest homology with osm1, hog1-homologue from Magnaporthe grisea. A growth defect was observed in cpmk1-null mutant under hyperosmotic conditions, indicating functionally belongs to hog1 subfamily. Immunoblot analyses indicated CpMK1 pathway affected specifically conditions hypovirus CHV1-EP713. Moreover, virus-infected hypovirulent UEP1 strain also exhibited severe osmosensitivity compared virus-free isogenic EP155/2, thus providing additional evidence for viral response hypertonic stress. Besides osmosensitivity, disruption resulted several, but not all, hypovirulence-associated changes, such as reduced pigmentation, conidiation, laccase production cryparin expression. However, an increased accumulation pheromone gene transcripts. Virulence assays canker area, UEP1. These results suggest mycoviruses modulate thereby provoke aberrant expression target genes, some are likely be implicated symptom development.