Brassinin Inhibits Proliferation in Human Liver Cancer Cells via Mitochondrial Dysfunction.

摘要: Brassinin is a phytochemical derived from Chinese cabbage, cruciferous vegetable. has shown anticancer effects on prostate and colon cancer cells, among others. However, its mechanisms hepatocellular carcinoma (HCC) have not been elucidated yet. Our results confirmed that brassinin exerted antiproliferative by reducing proliferating cell nuclear antigen (PCNA) activity, proliferation indicator inducing cycle arrest in human HCC (Huh7 Hep3B) cells. also increased mitochondrial Ca2+ levels depolarized the membrane both Huh7 Hep3B Moreover, generated high amounts of reactive oxygen species (ROS) lines. The ROS scavenger N-acetyl-L-cysteine (NAC) inhibited this brassinin-induced production. regulated AKT mitogen-activated protein kinases (MAPK) signaling pathways Furthermore, co-administering pharmacological inhibitors for JNK, ERK1/2 P38 decreased lines more than alone. Collectively, our demonstrated exerts via dysfunction MAPK pathway regulation