Factors involved in differential giemsa-staining of sister chromatids

摘要: Microspectrophotometric evaluation of differentially stained sister chromatids made it possible to analyse precisely the factors involved in Giemsa methods. The concentration Hoechst 33258, pH mounting medium, temperature during UV-exposure and quality (wavelength) UV-light influenced differential staining. Exposure blacklight 10−5 M 33528-stained BrdU-labeled chromosome specimens mounted McIlvaine buffer (pH 8.0) at 50° C reproducibly allowed staining within 15 min. On other hand, Korenberg-Freedlender's method using no 33258 was also UV-light-dependent. Thus, photolysis BrdU-substituted DNA considered basic mechanism methods where photosensitive played a role as sensitizer.