Anti-gamete monoclonal antibodies synergistically block transmission of malaria by preventing fertilization in the mosquito

摘要: Experiments from our laboratory previously demonstrated that infected chickens immunized with gametes of the avian malaria parasite Plasmodium gallinaceum were no longer infectious to mosquito vector Aedes aegypti and this transmission-blocking immunity was mediated by antibody. To identify those antigens are targets immunity, hybrid mouse cell lines secreting monospecific antibodies surface on male female P. have been produced. We describe two such anti-gamete antibodies, 10G3 11C7, which act synergistically both agglutinate in vitro suppress infectivity parasitized blood fed mosquitoes. In presence a mixture these became agglutinated during gametogenesis failed detach residual body gametocyte. either antibody alone, readily detached gametogenesis. Neither alone more than slight reduction gametocytes Although 11C7 recognize gametes, agglutination prevention detachment appears be primary mechanism prevents fertilization.