Ratiometric fluorogenic determination of endogenous hypochlorous acid in living cells
作者： Jiwen Hu , Xin Zhang , Tingting Liu , Hong-Wen Gao , Senlin Lu
DOI: 10.1016/J.SAA.2019.04.024
关键词: Oxadiazole 、 Detection limit 、 Selectivity 、 Linker 、 Fluorescence 、 Rhodamine 、 Coumarin 、 Photochemistry 、 Chemistry 、 Hypochlorous acid
摘要: Abstract Hypochlorous acid (HClO) is one of the most important ROS (reactive oxygen species) and common pollutant in tap-water. However, determination HClO with fast response high sensitivity/selectivity still an urgent demanding. Here we fabricated a ratiometric fluorescent probe RC based on TBET (through-bond energy transfer) platform coumarin rhodamine thiosemicarbazide group as linker. This could display characteristic fluorescence emission coumarin. Upon addition HClO, linker was reacted into oxadiazole, resulting opening spiro-ring rhodamine. The resultant then gives fluorogenic changes. exhibits selectivity sensitivity towards low limit detection (~140 nM). Eventually, successfully applicated for determining spiked water samples imaging endogenous living cells.
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