Transcriptional regulation of the neuronal L-type calcium channel α1D subunit gene
作者： Timothy J. Kamp , Michael Mitas , Kay L. Fields , Sadamitsu Asoh , Hemin Chin
DOI: 10.1007/BF02089942
关键词: Gene 、 Enhancer 、 Biology 、 Exon 、 Northern blot 、 Intron 、 Transcriptional regulation 、 Molecular biology 、 Reporter gene 、 Regulation of gene expression
摘要: 1. The transcriptional regulation of the rat brain L-type calcium channel α1D subunit (RBα1D) gene was investigated using NG108-15 neuroblastoma-glioma cells. 2. Differentiation cells in presence prostaglandin E1 or retinoic acid resulted appearance mRNA encoding RBα1D detected Northern blot analysis. 3. A genomic DNA library screened, and a 15.2-kb clone isolated partially sequenced which included part 5′ upstream sequence through initial intron 2 gene. 4. Deletion analysis, CAT reporter transfected cells, revealed that 1.2-kb 5′-upstream from contains cis-acting positive negative regulatory elements. deletion 3′ end exon 1 also suggested elements first exon. 5. DNase footprinting two regions protected digestion by specific protein binding, second region an (ATG)7 trinucleotide repeat sequence. Electrophoretic mobility shift assays confirmed nuclear protein(s) binding to 6. functions as enhancer when linked thymidine kinase promoter 7. These results provide description identify novel consists
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