The glucose metabolic distribution in thermophilic (55 °C) mixed culture fermentation: A chemostat study
作者： Fang Zhang , Yun Chen , Kun Dai , Nan Shen , Raymond J. Zeng
DOI: 10.1016/J.IJHYDENE.2014.11.098
关键词: Thermophile 、 Ethanol 、 Formate 、 Chemostat 、 Biochemistry 、 Propionate 、 Hydrogen 、 Chromatography 、 Fermentation 、 Metabolite 、 Chemistry
摘要: Abstract Thermophilic (55 °C) mixed culture fermentation (MCF) is a promising technology for converting organic wastes into bioproducts including hydrogen and chemicals. In this study, the effect of pH, partial pressure ( P H 2 ) influent glucose concentration on metabolic distribution in thermophilic chemostat was investigated. The results showed that metabolite composition changed from acetate, butyrate to ethanol, propionate format when pH increased 4.0 7.0. conversion formate found be thermodynamically controlled. Reducing did not significantly increase yield consequently metabolites change much. Lactate accumulation observed only at high concentration. maximum 2.2 ± 0.16 mol/mol-glucose 0.05 atm, while production rate 7.9 ± 0.1 L/(L-reactor·Day) 76.3 mmol/L. Thus, considering operating cost, increasing 76.3 mmol/L work could more practical approach produce MCF. Meanwhile, impacts precedent were negligible chemostat. thermodynamic analysis illustrated mixture butyrate, and/or lactate essential producing
elsevier.com
sci-hub.se 参考文章(30)
A E Greenberg, Franson M.A.H., L S Clesceri, A D Eaton, Standard methods for the examination of water and wastewater. 19th ed. 1995. ,(1995)
Andrew Eaton, A.E. Greenberg, Lenore S. Clesceri, Standard methods for the examination of water and wastewater Published in <b>1998</b> in Washington DC) by American public health association. ,(1992)
Armen Trchounian, Escherichia coli proton-translocating F0F1-ATP synthase and its association with solute secondary transporters and/or enzymes of anaerobic oxidation-reduction under fermentation. Biochemical and Biophysical Research Communications. ,vol. 315, pp. 1051- 1057 ,(2004) , 10.1016/J.BBRC.2004.02.005
Jorge Rodríguez, Robbert Kleerebezem, Juan M. Lema, Mark C.M. van Loosdrecht, Modeling product formation in anaerobic mixed culture fermentations. Biotechnology and Bioengineering. ,vol. 93, pp. 592- 606 ,(2006) , 10.1002/BIT.20765
Patrick C Hallenbeck, John R Benemann, Biological hydrogen production; fundamentals and limiting processes International Journal of Hydrogen Energy. ,vol. 27, pp. 1185- 1193 ,(2002) , 10.1016/S0360-3199(02)00131-3
Hana Gannoun, Nada Ben Othman, Hassib Bouallagui, Hamdi Moktar, Mesophilic and thermophilic anaerobic co-digestion of olive mill wastewaters and abattoir wastewaters in an upflow anaerobic filter Industrial & Engineering Chemistry Research. ,vol. 46, pp. 6737- 6743 ,(2007) , 10.1021/IE061676R
Karin Willquist, Ed W.J. van Niel, Lactate formation in Caldicellulosiruptor saccharolyticus is regulated by the energy carriers pyrophosphate and ATP. Metabolic Engineering. ,vol. 12, pp. 282- 290 ,(2010) , 10.1016/J.YMBEN.2010.01.001
Y. Lu, F. R. Slater, Z. Mohd-Zaki, S. Pratt, D. J. Batstone, Impact of operating history on mixed culture fermentation microbial ecology and product mixture Water Science and Technology. ,vol. 64, pp. 760- 765 ,(2011) , 10.2166/WST.2011.699
I.-Chun Liu, Liang-Ming Whang, Wei-Jie Ren, Pei-Ying Lin, The effect of pH on the production of biohydrogen by clostridia: Thermodynamic and metabolic considerations International Journal of Hydrogen Energy. ,vol. 36, pp. 439- 449 ,(2011) , 10.1016/J.IJHYDENE.2010.10.045
Rodrigo A. Labatut, Largus T. Angenent, Norman R. Scott, Conventional mesophilic vs. thermophilic anaerobic digestion: a trade-off between performance and stability? Water Research. ,vol. 53, pp. 249- 258 ,(2014) , 10.1016/J.WATRES.2014.01.035