3,3′-Diamino-N-methyldipropylamine as a versatile affinity ligand

摘要: Currently, in biomedicine and biotechnology fields, there is a growing need to develop produce biomolecules with high degree of purity. To accomplish this goal, new purification methods are being developed looking for higher performance, efficiency, selectivity, cost-effectiveness. Affinity chromatography considered one the most highly selective purification. The purpose work explore type structurally simple ligand immobilized onto an agarose matrix be used affinity chromatography. study, 3,3′-diamino-N-methyldipropylamine has shown low toxicity cost preparation. Moreover, ability purify proteins nucleic acids was verified. An increasing sodium chloride gradient, using salt concentrations up 500 mM, suitable these biomolecules, meaning that support allows recovery target under mild conditions. Thus, useful versatile tool chromatographic experiments, very good results either or supercoiled plasmid isoform