Acetylcholinesterase Inhibition-Based Biosensor for Aluminum(III) Chronoamperometric Determination in Aqueous Media
作者： Miriam Barquero-Quirós , Olga Domínguez-Renedo , Maria Alonso-Lomillo , María Arcos-Martínez
DOI: 10.3390/S140508203
关键词: Amperometry 、 Biosensor 、 Substrate (chemistry) 、 Reproducibility 、 Colloidal gold 、 Nuclear chemistry 、 Tap water 、 Analytical chemistry 、 Detection limit 、 Chemistry 、 Conductometry
摘要: A novel amperometric biosensor for the determination of Al(III) based on inhibition enzyme acetylcholinesterase has been developed. The immobilization was performed screen-printed carbon electrodes modified with gold nanoparticles. oxidation signal acetylthiocholine iodide substrate affected by presence ions leading to a decrease in current. developed system detection limit 2.1 ± 0.1 μM Al(III). reproducibility method is 8.1% (n = 4). Main interferences include Mo(VI), W(VI) and Hg(II) ions. successfully applied spiked tap water . analysis certified standard reference material also carried out. Both results agree values considering respective associated uncertainties.
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