Molecular-Genetic Biodiversity in a Natural Population of the Yeast Saccharomyces cerevisiae From “Evolution Canyon”: Microsatellite Polymorphism, Ploidy and Controversial Sexual Status
作者： T. Katz Ezov , E. Boger-Nadjar , Z. Frenkel , I. Katsperovski , S. Kemeny
DOI: 10.1534/GENETICS.106.062745
关键词: Saccharomyces cerevisiae 、 Polyploid 、 Microsatellite 、 Natural population growth 、 Evolutionary biology 、 Ploidy 、 Genetic diversity 、 Genetics 、 Phylogenetics 、 Genetic variation 、 Biology
摘要: The yeast S. cerevisiae is a central model organism in eukaryotic cell studies and major component many food biotechnological industrial processes. However, the wide knowledge regarding genetics molecular biology of based on an extremely narrow range strains. Studies natural populations cerevisiae, not associated with human activities or fermentation environments, are very few. We isolated panel strains from microsite, “Evolution Canyon” at Mount Carmel, Israel, studied their genomic biodiversity. Analysis 19 microsatellite loci revealed high allelic diversity variation ploidy level across panel, diploids to tetraploids, confirmed by flow cytometry. No significant differences were found between derived localities microniches, whereas different showed low similarity allele content. Maximum genetic was observed among minimum triploids. Phylogenetic analysis clonal, rather than sexual, structure triploid tetraploid subpopulations. Viability tests tetrad also suggest that clonal reproduction may predominate polyploid
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