Contribution of 13C-NMR spectroscopy to the elucidation of pathways of propionate formation and degradation in methanogenic environments

摘要: Propionate is an important intermediate in the anaerobic degradation of complex organic matter to methane and carbon dioxide. The metabolism propionate-forming propionate-degrading bacteria reviewed here. formed during fermentation polysaccharides, proteins fats. study fate 13C-labelled compounds by nuclear magnetic resonance (NMR) spectroscopy has contributed together with other techniques present knowledge metabolic routes which lead propionate formation from these substrates. Since oxidation under methanogenic conditions thermodynamically difficult, often accumulates when rates its are unbalanced. Bacteria able degrade substrates acetate hydrogen can only perform this reaction methanogens consume efficiently. As a consequence, be degraded obligatory syntrophic consortia microorganisms. NMR were used defined less cultures consortia. Different types side-reactions reported, like reductive carboxylation butyrate acetylation higher fatty acids.