Byrsonima crassifolia extract and fraction prevent UVB-induced oxidative stress in keratinocytes culture and increase antioxidant activity on skin

摘要: Abstract Solar ultraviolet radiation exposure, particularly UVB rays (280–320 nm), can lead to skin lesions, photocarcinogenesis and acceleration of photoaging since may reach both the epidermal dermal layers skin. Treatments that ameliorate UVB-induced damage include natural extracts, which act as photochemoprotective agents. Byrsonima crassifolia is widely used in folk medicine. Previously studies have shown high antioxidant activity BC leaves extracts. In this study we described first potential extract (BCP) fraction (BCF) against keratinocytes ability topical formulations with BCP or BCF increase pig ear The results characterization indicate phenolic content was increased two-fold after an enrichment process for obtaining BCF. Despite differences content, exhibited similar IC50 values lipid peroxidation DPPH method during study. However, chemiluminescence assay using xanthine/luminol/XOD, higher than BCP. different did not influence their HaCaT cells, samples levels protection. After treatment (1.2–5 μg/mL) irradiation effect vitro maintained cell culture, IL-6 TNF-α secretion NF-κB activation were suppressed. development formulations, on formulation containing showed retention, especially (+) catechin, able pass through stratum corneum. Based these findings, could be topically applied prevent/treat induced by