Isolation, purification, characterization and immunostimulatory activity of polysaccharides derived from American ginseng.
作者： Xiao-Hong Yu , Ying Liu , Xian-Ling Wu , Li-Zhai Liu , Wei Fu
DOI: 10.1016/J.CARBPOL.2016.08.092
关键词: Nuclear magnetic resonance spectroscopy 、 Chromatography 、 American ginseng 、 Polysaccharide 、 Chemistry 、 Gas chromatography 、 In vitro 、 Size-exclusion chromatography 、 Chemical engineering 、 Sepharose 、 Nitric oxide
摘要: In this study, crude American ginseng polysaccharide (AGPS) was extracted with hot water and preliminarily purified by using resin S-8 and Polyamide columns. Then, it was further purified and separated by DEAE-Sepharose CL-6B and Sepharose CL-6B chromatography, respectively. Five main fractions were obtained, named WPS-1, WPS-2, SPS-1, SPS-2 and SPS-3. Their homogeneities and structural characteristics were elucidated based on UV–vis spectroscopy, High Performance Gel Filtration Chromatography (HPGFC), Gas …
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