Effects of insulin-like growth factor I and insulin on proliferation and on basal and luteinizing hormone-induced steroidogenesis of bovine thecal cells: involvement of glucose and receptors for insulin-like growth factor I and luteinizing hormone.
作者： R E Stewart , L J Spicer , T D Hamilton , B E Keefer
关键词: Luteinizing hormone 、 Gonadotropin 、 Biology 、 Internal medicine 、 Androstenedione 、 Ovarian follicle 、 Insulin-like growth factor 、 Insulin 、 Endocrinology 、 Theca 、 Follicular phase
摘要: The objective of the present study was to determine effects IGF-I and insulin on cell proliferation, LH receptors, basal LH-induced progesterone androstenedione production by bovine thecal cells. Cells from large (> or = 8mm) follicles were cultured for 1 2 d in medium containing 10% fetal calf serum (FCS) treated serum-free with IGF-I, insulin, and(or) LH. Treatment 30 100 ng/mL increased numbers absence regardless whether treatments initiated after exposure FCS. Co-treatment reduced stimulatory effect numbers. Insulin at 10 presence Both ineffective stimulating However, (P .10) binding sites cells but < .05) production. <.01) LH/hCG sites. These results indicate that may each play a significant role mitogenesis steroidogenesis during follicular development cattle glucose enhances these effects. Furthermore, synergism between increasing does not seem be mediated through rather, part,
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