Anti-HIV activity against immunodeficiency virus type 1 (HIV-I) and type II (HIV-II) of compounds isolated from the stem bark of Combretum molle.

摘要: Abstract In vitro anti-HIV activity of various extracts prepared from the stem bark Combretum molle (R. Br. Ex. G. Don.) Engl & Diels (Combretaceae), a plant widely used in Ethiopian traditional medicine for treatment liver diseases, malaria and tuberculosis has been assessed against human imnmuunodeficiencvy virus type 1 (HIV-1) 2 (HIV-2). The total extract was by percolation with 80% methanol whilst petroleum ether, chloroform, acetone 100% fractions were obtained successive hot extraction using Soxhlet apparatus. Selective inhibition viral growth simultaneous determination cytotoxicity each MT-4 cells. Results this study indicate that fraction possessed highest selective HIV-1 replicatuon. Phytochemical investigation resulted isolation two tannins oleanane-type pentacyclic triterpene glycosides. One identified as puncalagin (an ellagitannin), structure other (CM-A) not yet fully elucidated. saponins characterized arjunglucoside (also called 4-epi-sericoside) sericoside did inhibit replication either or HIV-2. On hand, both punicalgin CM-A displayed selectrvitv indices (ratio 50% cytotoxic concentration to effective antiviral concentration) 16 25, respectivelvy afforded cell protection induced cytopathic effect when compared control samples. Neither exhibited HIV-2 at nontoxic doses.