Application of laser printing to mammalian cells
作者： Jason A. Barron , Bradley R. Ringeisen , Heungsoo Kim , Barry J. Spargo , Douglas B. Chrisey
DOI: 10.1016/J.TSF.2003.11.161
关键词: Evaporation (deposition) 、 Tissue engineering 、 Laser 、 Laser printing 、 Materials science 、 Maple 、 Nanotechnology 、 Biopolymer 、 Cytotoxicity 、 Matrix (biology)
摘要: Abstract A laser-based transfer technique, termed matrix assisted pulsed laser evaporation direct write (MAPLE DW), has been modified to rapidly and accurately deposit mammalian cells in a non-contact manner. While this technology was originally developed for deposition of inorganic materials, it shown the ability wide range biological materials. Two types cells, human osteosarcoma rat cardiac were deposited into biopolymer via MAPLE DW. Current results show that is possible stepwise manner build cellular ‘stacks’ 50–100 μm tall. Furthermore, technique now capable depositing with near single cell resolution. Post-transfer live/dead viability/cytotoxicity assays are unaffected by process 100% viability. The structures three dimensions small numbers potential application tissue engineering.
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