Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dynamics of the Serotonin1A Receptor

摘要: G protein-coupled receptors (GPCRs) are the largest class of molecules involved in signal transduction across membranes and represent major targets development novel drug candidates all clinical areas. Recent advances understanding nonrandom distribution GPCRs, proteins, effector have given rise to new challenges complexities cellular signaling by GPCRs. In this article, we provide specific examples on application quantitative fluorescence microscopic approaches monitor organization dynamics serotonin1A receptor (a GPCR) live cells. This assumes broader relevance due emerging theme that GPCR function depends its dynamics. We envisage with progress dynamics, our knowledge would improve considerably, thereby enabling design better therapeutic strategies combat diseases related malfunctioning