Optical properties of artificial chlorophyll membranes.

摘要: The composition and structure of lipid bilayer membranes containing chlorophylla have been studied with photometric fluorometric methods. A sensitive double-beam spectrophotometer is described by which the pigment concentration in can be determined. Up to 3×1013 chlorophyll molecules per cm2 incorporated into membrane, corresponding a mean distance 20 between porphyrin rings. At high concentrations, absorption peaks are shifted toward longer wavelengths, indicating an interaction rings film. Parallel spectral shifts, large decrease fluorescence quantum yield depolarization observed. These findings suggest that transfer excitation energy takes place neighboring membrane. When oxidating agent (K2S2O8) added toone external phase, exactly half film destroyed. This observation suggests localized membrane surfaces phytyl chains inserted hydrocarbon core facing aqueous solution.