Detergent-assisted refolding of guanidinium chloride-denatured rhodanese. The effects of the concentration and type of detergent.

摘要: We have established the generality of using detergents for facilitating reactivation 6 M guanidinium chloride-denatured rhodanese that was recently described nonionic detergent lauryl maltoside (LM) (Tandon, S., and Horowitz, P. (1986) J. Biol. Chem. 261, 15615-15618). report here not only LM but other as well ionic zwitterionic also favorable effects in reactivating denatured enzyme. Not all are useful, occur over a limited concentration range. Above below range there is little or no effect. Zwittergents, which represent homologous series with varying critical micelle concentrations (CMCs) effective above their CMCs. Induction phases progress curves refolded presence detergents, suggesting refolding intermediates apparently stabilized by interactions. Gel filtration chromatography without suggests even though renaturation enzyme requires at its CMC, does bind an amount equivalent to micelle. It suggested proteins might be assisted inclusion "nondenaturing" although optimal conditions will determined each individual case.