Regulation of the streptokinase-mediated activation of human plasminogen by fibrinogen and chloride ions.

摘要: The effects of human fibrinogen and Cl- on the activation native plasminogen by streptokinase, at 37 degrees C, in isotonic buffers, consisting 10 mM Hepes-NaOH, 150 NaOAc-NaCl, pH 7.4, have been examined. We find that acts as a mixed-type inhibitor activation, with Ki range 6.4-9.2 mM, absence presence (1.0 microM) fibrinogen. Fibrinogen displays an effect kinetics consistent its function nonessential activator, Ka values between 110 240 nM, (50 mM) Cl-. These observations suggest opposing roles for regulating activation.