MicroRNA-25 regulates small cell lung cancer cell development and cell cycle through cyclin E2.

摘要: Purpose: We intended to examine the underlying mechanism of microRNA-25 (miR-25) in regulating small cell lung cancer (SCLC). Methods: The miR-25 expression was measured by quantitative RT-PCR (qRT-PCR) 5 SCLC lines and 9 human tissues. In line H510A cells, endogenous downregulated stable transfection antisense oligonucleotide (miR-25-as). Then effects downregulation on growth, invasion chemoresistance were assessed MTT, migration cisplatin assays, respectively. Furthermore, cycle arrest, production proteins cyclin E2 CDK2 examined assay, western blot luciferase Finally, over-expressed cells investigate its effect mediated regulation. Results: both tumor tissues, overexpressed. Down-regulation significantly reduced invasive capability resistance cisplatin. Also, it induced G1 arrest related CDK2. Luciferase assay demonstrated directly targeted miR-25. Overexpression reversed restored impaired downregulation. Conclusions: Our study shows is overexpressed acting as oncogenic regulator E2.