Studies on the assembly of a spherical plant virus

摘要: Potentiometric and spectrophotometric titrations have been undertaken to understand the mechanisms of control pH-dependent swelling cowpea chlorotic mottle virus formation capsid by viral protein. The results show a striking difference in proton release virion Both are accompanied an anomalous which shows well-defined hysteresis pH range where these phenomena take place. is not any major change u.v. absorption, but only small decrease (2% at 260 nm). Magnesium ions, marked effect on swelling, no protein titration, abolish suppress absorbance associated with swelling. The data suggest that two carboxyl groups pK near neutrality responsible for controlling also probably assembly. magnesium ions presumably nucleic acid configuration (or) RNA-protein salt links. The existence hysteresis, both capsids from virus, interesting feature, showing pathway through metastable states.